Composite

Part:BBa_K1972014:Design

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-10-14)


dszBBAC with tac promoter and dszD with lac promotor induced by IPTG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4052
    Illegal XhoI site found at 5558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 1582
    Illegal NgoMIV site found at 3076
    Illegal AgeI site found at 1104
    Illegal AgeI site found at 2419
    Illegal AgeI site found at 3180
    Illegal AgeI site found at 3463
    Illegal AgeI site found at 4281
    Illegal AgeI site found at 5746
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 864
    Illegal BsaI.rc site found at 2179


Design Notes

Not yet.


Source

Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1) The T7 RNA polymerase controled system is PCRed from E.coli Bl21.

References